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Dr. Kazuhiko Narisawa

Dr. Kazuhiko Narisawa

Ibaraki University, Japan

Title: Establishment of plant vaccinating system by a dark septate endophytic fungus (DSE) - DSE fungi alter the root-associated microbial communities and suppress soil borne diseases of plant

Biography

Biography: Dr. Kazuhiko Narisawa

Abstract

Crop production has been dependent on chemicals to control pest organisms and supply nutrients for growth. These chemicals have caused considerable negative impacts on agricultural soils since their introduction. It is today’s aim to reduce the use of chemicals and switch to a biological control method for pest control purposes, i.e., create a natural and organic agricultural system. However, the scientific basis for many biocontrol methods, which proves the validity of the organic production system, is insufficient. Fusarium crown and root rot (FCRR) of tomato caused by Fusarium oxysporum f. sp. radicis-lycopersici (FORL) is a common disease observed worldwide. Our Previous study showed the suppressive role of a DSE fungus, Veonaeopsis simplex Y34 against Fusarium disease of Chinese cabbage, suggesting a potential bio-control agent to suppress FCRR of tomato. The solid-substrate, incubated by combining V. simplex Y34 with sterilized litter compost (solid-cultures of V. simplex Y34), was applied in a nursery pot to assess the bio-control of the disease. Results showed that V. simplex Y34 decreased the disease severity of FCRR for the two cultivars. The colonization of V. simplex Y34 in root was determined by re-isolation and terminal-restriction fragment length polymorphism (T-RFLP) analysis targeting fungal ITS-LSU region. Moreover, the application of the endophyte increased the diversity of the fungal community in root-endosphere and decreased the colonization of FORL in the root. The T-RFs-based multivariate analysis showed that the fungal communities in root-endosphere with inoculation of V. simplex Y34 were clustered away from those of non-inoculation, suggesting a correlation between the root-endospermic community and disease incidence. In conclusion, this study indicates that the application of V. simplex Y34 altered the diversity, evenness, and structure of root-endospermic fungal community by the endosymbiosis of endophyte, and decreased pathogen colonization in the root, which opens a new way to control of tomato FCRR disease.