Dr. M Harun-or Rashid
Bangladesh Institute of Nuclear Agriculture, Bangladesh Panel"
Title: Isolation and evaluation of bacterial strains with antifungal activity against the phytopathogenic fungi
Biography
Biography: Dr. M Harun-or Rashid
Abstract
To feed the ever-increasing population and sustainable agriculture, more-efficient control of plant diseases is essential. The use of naturally occurring bacterial antagonists that suppress pathogens has recently reemerged as a promising alternative to use of agrochemical for controlling fungal diseases. A total of 40 bacterial strains were isolated from stem and leaf of maize and rice and, soil to find effective bacterial strains with antifungal activity against major fungal diseases of rice and wheat. We evaluated bio-control potentiality of isolated bacterial strains against the major fungal pathogen of rice, wheat, and sugarcane viz. Bipolaris oryzae, Pyricularia oryzae, Magnaporthe oryzae, Bipolaris sorokiniana and Colletotrichum falcatum at the in-vitro condition. Out of forty, sixteen bacterial strains suppressed the tested fungal pathogens from 25% to 100% under in vitro plate assay. These strains were characterized by DNA fingerprinting which formed two major groups among sixteen strains. Out of sixteen seven strains were very close to Bacillus sp. A pot experiment was conducted at field conditions by artificial inoculation of wheat using fungal pathogen Bipolaris sorokiniana and selected bacterial strains were used as bio-control agents along with chemical fungicide (Bactroban). Inoculation experiment revealed that the strain GO-1 showed the highest bio-control potential against leaf blight of wheat caused by Bipolaris sorokiniana. It showed lower disease incidence (33%) and greater seed yield (4 g/plant) while control treatment had the incidence of the maximum disease (87%) and the lowest seed yield (2 g/plant). Sequencing of 16S rRNA gene, screening for bio-control traits and plant growth promoting traits of selected strains are in progress.